| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.162566 |
| Chromosome: | chromosome 6 |
| Location: | 1112003 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g256750 | FAT1,TEH9 | Acyl carrier protein thioesterase; (1 of 1) K10782 - fatty acyl-ACP thioesterase A (FATA) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCCATCACAGCCACGAACTCTACTCAGC |
| Internal bar code: | TATGGCCGGTGGGCAACCGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 725 |
| LEAP-Seq percent confirming: | 89.7666 |
| LEAP-Seq n confirming: | 500 |
| LEAP-Seq n nonconfirming: | 57 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGTGATGTTGGCGTGTTC |
| Suggested primer 2: | GAGGTCATTCGGTGAGCATT |