| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.162585 |
| Chromosome: | chromosome 3 |
| Location: | 5881425 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g189700 | (1 of 2) K07735 - putative transcriptional regulator (algH) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGGGAAACTTGATGGGTGACGAGAGAGG |
| Internal bar code: | ACTTCATTCTCGCTGCACTAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 270 |
| LEAP-Seq percent confirming: | 99.0573 |
| LEAP-Seq n confirming: | 1366 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGCTGCTTTACTACCCGC |
| Suggested primer 2: | GATCGGCATCAACGGTTAGT |