| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.162592 |
| Chromosome: | chromosome 6 |
| Location: | 5487326 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g284700 | AAT2 | Alanine aminotransferase; (1 of 1) K00814 - alanine transaminase (GPT, ALT) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACAACCTCCCGCCTCCTCGCAACCTGTGC |
| Internal bar code: | GGGGATGCAATTTAACATCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 323 |
| LEAP-Seq percent confirming: | 99.6793 |
| LEAP-Seq n confirming: | 4040 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTATGCCCTGTGTTTTGTG |
| Suggested primer 2: | GATGTTAGCGCAGGTTTGGT |