| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.162598 |
| Chromosome: | chromosome 13 |
| Location: | 4043169 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g591150 | HTA38,HTA22 | Histone H2A; (1 of 30) K11251 - histone H2A (H2A) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCGGATCCCCCAATTTCCTGAATCTCCT |
| Internal bar code: | GAGTTGCGCACTTATAGCGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 995 |
| LEAP-Seq percent confirming: | 98.2934 |
| LEAP-Seq n confirming: | 4435 |
| LEAP-Seq n nonconfirming: | 77 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCTCCTGTGAGTCTGGAT |
| Suggested primer 2: | CGTACAATGAAACAATGCGG |