| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.162607 |
| Chromosome: | chromosome 1 |
| Location: | 2117017 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g011450 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTAGATCCGCAAGGTAGGGTTAAGGTAGA |
| Internal bar code: | AGGTCACGAGACTTCGGAACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 324 |
| LEAP-Seq percent confirming: | 89.6775 |
| LEAP-Seq n confirming: | 7619 |
| LEAP-Seq n nonconfirming: | 877 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGGGTGGTTATCATGGTG |
| Suggested primer 2: | TCTCTGTGTATTGCAACGGC |