| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.162611 |
| Chromosome: | chromosome 17 |
| Location: | 6279708 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g742500 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTCAGCACGGCGCCCTTTTCCCACCACA |
| Internal bar code: | CTAGCGGTCGGACGTGTCACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1075 |
| LEAP-Seq percent confirming: | 99.7835 |
| LEAP-Seq n confirming: | 2765 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGTCGGTGTGCGTTACTA |
| Suggested primer 2: | CTATGTACGTTCGGCCCACT |