| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.162612 |
| Chromosome: | chromosome 5 |
| Location: | 318512 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g233400 | (1 of 52) 2.7.11.22 - Cyclin-dependent kinase / Cdk-activating protein kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTGTATAAGGCGATCAGCTGCCTAGAGC |
| Internal bar code: | GGTTGCCATTAGGGGTTGGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 848 |
| LEAP-Seq percent confirming: | 95.9391 |
| LEAP-Seq n confirming: | 3024 |
| LEAP-Seq n nonconfirming: | 128 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATCTACGCAATCCTCCAT |
| Suggested primer 2: | GCTGCAGTGTGTCATTGCTT |