| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.162612 |
| Chromosome: | chromosome 14 |
| Location: | 2921400 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g627576 | DHC7 | (1 of 2) PTHR10676//PTHR10676:SF136 - DYNEIN HEAVY CHAIN FAMILY PROTEIN // DYNEIN HEAVY CHAIN 6, AXONEMAL; Axonemal Dynein Heavy Chain 7 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGCTGCTGCCCACACCCGCCAAGAGCC |
| Internal bar code: | TTACCCCTGGCTTCATTTCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 481 |
| LEAP-Seq percent confirming: | 99.7498 |
| LEAP-Seq n confirming: | 25912 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCAAACAGCTCCTCATAGC |
| Suggested primer 2: | GATTCTGGCCTCACGTTCAT |