| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.162618 |
| Chromosome: | chromosome_2 |
| Location: | 2803228 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g094350 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGCCCCCCAGCCGTTTAAAATGGGGAGGGG |
| Internal bar code: | GGAAGCCATGGAACGGGGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 358 |
| LEAP-Seq percent confirming: | 99.4475 |
| LEAP-Seq n confirming: | 720 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTGAGAGTGGATTGCTCC |
| Suggested primer 2: | TTTTGGTATTGTGCCGTGAA |