Insertion junction: LMJ.RY0402.162624_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.162624
Chromosome:chromosome 7
Location:1811295
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g325727 (1 of 3) PTHR10566:SF76 - PROTEIN Y32H12A.7 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGCTCAAAAAGGCGGAAAACGTCGCAAGG
Internal bar code:TTCCGGCGCCGGCTTCCATGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:722
LEAP-Seq percent confirming:99.6332
LEAP-Seq n confirming:4074
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:GTAATGGTGTGCACTGACGG
Suggested primer 2:GGTTTGCTGGGTGTACAGGT