| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.162627 |
| Chromosome: | chromosome 17 |
| Location: | 1715518 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g708850 | (1 of 226) IPR012337 - Ribonuclease H-like domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGTGTTGAGCCTCGGAAGATGAAACGGC |
| Internal bar code: | TAATATGTTCTCTTAGGTGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 159 |
| LEAP-Seq percent confirming: | 91.0853 |
| LEAP-Seq n confirming: | 235 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTGCTGAAGTCTAGGGTG |
| Suggested primer 2: | GGATGCTACAGCAACAGCAA |