Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.162644 |
Chromosome: | chromosome_10 |
Location: | 350154 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g420450 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CAACTTCATGATGCAGCTCAAGGTGCGGCT |
Internal bar code: | AGCTTATGCGGCATCCTCCACA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 158 |
LEAP-Seq percent confirming: | 90.9091 |
LEAP-Seq n confirming: | 60 |
LEAP-Seq n nonconfirming: | 6 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTGGTCCACATCATCACTGC |
Suggested primer 2: | AAGCATCCACACAATGGACA |