| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.162671 |
| Chromosome: | chromosome 9 |
| Location: | 5834291 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g402256 | (1 of 3) 3.4.11.1 - Leucyl aminopeptidase / Peptidase S | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGACCATCGCGCGCGACTTATTCGGCACG |
| Internal bar code: | ATTGCACCGATCGCTTGCTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1037 |
| LEAP-Seq percent confirming: | 99.8022 |
| LEAP-Seq n confirming: | 1514 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTTGTACATGGTACGGCA |
| Suggested primer 2: | CAGGGATGGATCAGGAGAAA |