| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.162673 |
| Chromosome: | chromosome 11 |
| Location: | 2123970 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g469375 | (1 of 41) PTHR32523//PTHR32523:SF8 - FAMILY NOT NAMED // PHYTOL KINASE 1, CHLOROPLASTIC | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCAAGGGCTCAGCGGCGGGCCCCTCAAT |
| Internal bar code: | AAGGCCATCTAGACAGGGAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 273 |
| LEAP-Seq percent confirming: | 99.8049 |
| LEAP-Seq n confirming: | 5115 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGCAATAGCTCGTAGAAG |
| Suggested primer 2: | GTGGATGTGAATGGAAAGGG |