| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.162685 |
| Chromosome: | chromosome 12 |
| Location: | 3436456 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g496400 | (1 of 2) 2.7.7.52 - RNA uridylyltransferase / TUT | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACCATCCAGCACAACAATATTCGCACCA |
| Internal bar code: | AGGTAGACCTCTCGAGCGTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 409 |
| LEAP-Seq percent confirming: | 98.9122 |
| LEAP-Seq n confirming: | 21822 |
| LEAP-Seq n nonconfirming: | 240 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCTGGCCTGAGGTAGTTG |
| Suggested primer 2: | CCAGACATTGAACGGACCTT |