| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.162711 |
| Chromosome: | chromosome 17 |
| Location: | 2899562 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g719701 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCGCTGTTATCCGTCTGCACCGCTTTGG |
| Internal bar code: | ATTGTGCTTAGGGGCAGGCCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1058 |
| LEAP-Seq percent confirming: | 99.3261 |
| LEAP-Seq n confirming: | 8844 |
| LEAP-Seq n nonconfirming: | 60 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGTAGGCCGAAACCACTG |
| Suggested primer 2: | TATGAGCTTGACGCGTTGAC |