| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.162727 |
| Chromosome: | chromosome 4 |
| Location: | 3069952 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g225350 | (1 of 3) IPR002483 - PWI domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAGAGTGAGATGTGGACGCAGCCGATGT |
| Internal bar code: | CTAATAGCGAGCAGCAATTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 807 |
| LEAP-Seq percent confirming: | 99.6133 |
| LEAP-Seq n confirming: | 4379 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAAATAAAGGTTCAGGGCA |
| Suggested primer 2: | GGGAGGAGAACGTTTAAGGC |