Insertion junction: LMJ.RY0402.162732_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.162732
Chromosome:chromosome_12
Location:4195480
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre12.g518550 FAP54 Flagellar Associated Protein antisense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):TGGGGTTGGAGCGCCACAGCGGCACGTCCG
Internal bar code:AAGCAGTCTGGTCGGGGAGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:63
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:27
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TTGGTTGTTCTACCCCCGTA
Suggested primer 2:CCTGCTTGTAAAGGCTCAGG