| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.162762 |
| Chromosome: | chromosome 12 |
| Location: | 6882976 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g560950 | PSAG1,PSAG | (1 of 1) K08905 - photosystem I subunit V (psaG); Photosystem I reaction center subunit V | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAATCGCAAGTGCATTTGCTTCTACCGTGG |
| Internal bar code: | TGTACAGCAGTCCCACAAATTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 475 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCCTCCTTATGGTAGTCA |
| Suggested primer 2: | AAGCTCGGTCCACTGTCTGT |