| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.162766 |
| Chromosome: | chromosome 10 |
| Location: | 1712507 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g430450 | (1 of 1) 3.5.1.10 - Formyltetrahydrofolate deformylase / Formyltetrahydrofolate hydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTCAGGGAAAGGCTGAACAGGTCGGGAG |
| Internal bar code: | GACCGGCTCCGCATCGGCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1020 |
| LEAP-Seq percent confirming: | 99.5511 |
| LEAP-Seq n confirming: | 9536 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 73 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCTCAGTCCCGATCCCTA |
| Suggested primer 2: | GTCGTTCGGGCCTATAATCA |