| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.162773 |
| Chromosome: | chromosome 2 |
| Location: | 6743558 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g118400 | ZCP2 | Zn chaperone; (1 of 5) PTHR13748:SF4 - COBW DOMAIN-CONTAINING PROTEIN DDB_G0274527 | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGCACGTCGCGAACGGGCAACAAGCTGG |
| Internal bar code: | AGGGGTTATTACCAAAATTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 30.3227 |
| LEAP-Seq n confirming: | 1635 |
| LEAP-Seq n nonconfirming: | 3757 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGATGACGACAGCAGCAG |
| Suggested primer 2: | GTGTTATGTGCAGTTGCGCT |