Insertion junction: LMJ.RY0402.162781_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.162781
Chromosome:chromosome 11
Location:1269716
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g467705 (1 of 3) K02154 - V-type H+-transporting ATPase subunit a (ATPeV0A, ATP6N) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCATCATCTTCGGCCGCACCAAATTCAAA
Internal bar code:TATGGCGCGCACGGCCACGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:107
LEAP-Seq percent confirming:84.3972
LEAP-Seq n confirming:119
LEAP-Seq n nonconfirming:22
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GCCACCTACCTATATCCGCA
Suggested primer 2:CCATCCATTTTGCCTTTTGT