Insertion junction: LMJ.RY0402.162786_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.162786
Chromosome:chromosome 6
Location:1024853
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g256150 (1 of 19) 3.6.1.3 - Adenosinetriphosphatase / Triphosphatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTAGCGCCAGGGAAGGAGCACAAGGTAGA
Internal bar code:TTGTTCTCCTCTTGGCGCTAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:909
LEAP-Seq percent confirming:74.7788
LEAP-Seq n confirming:338
LEAP-Seq n nonconfirming:114
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:ACTTGACCTCAACCACCGTC
Suggested primer 2:GACCGGATCATGCGAATACT