Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.162787 |
Chromosome: | chromosome 14 |
Location: | 3265697 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre14.g629500 | (1 of 1) PF06244 - Protein of unknown function (DUF1014) (DUF1014) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGACCGTAATCACCACCATCGTCATCAT |
Internal bar code: | ACTGTCTGCCCGTCGGGCCACA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 441 |
LEAP-Seq percent confirming: | 46.2633 |
LEAP-Seq n confirming: | 130 |
LEAP-Seq n nonconfirming: | 151 |
LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CACACACCAACCTATCGCAC |
Suggested primer 2: | TGTCGGGAGGTGTGTGTAAA |