Insertion junction: LMJ.RY0402.162787_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.162787
Chromosome:chromosome 14
Location:3265697
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g629500 (1 of 1) PF06244 - Protein of unknown function (DUF1014) (DUF1014) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGACCGTAATCACCACCATCGTCATCAT
Internal bar code:ACTGTCTGCCCGTCGGGCCACA

Confirmation - LEAP-Seq

LEAP-Seq distance:441
LEAP-Seq percent confirming:46.2633
LEAP-Seq n confirming:130
LEAP-Seq n nonconfirming:151
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CACACACCAACCTATCGCAC
Suggested primer 2:TGTCGGGAGGTGTGTGTAAA