| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.162787 |
| Chromosome: | chromosome 14 |
| Location: | 3265703 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g629500 | (1 of 1) PF06244 - Protein of unknown function (DUF1014) (DUF1014) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATCCAAAGAAGAGAACAGGTGCGCAGGG |
| Internal bar code: | CCGTGATCATGCTCTTGTCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 135 |
| LEAP-Seq percent confirming: | 99.4835 |
| LEAP-Seq n confirming: | 963 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACACCAACCTATCGCAC |
| Suggested primer 2: | TGTCGGGAGGTGTGTGTAAA |