| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.162798 |
| Chromosome: | chromosome 12 |
| Location: | 7192208 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g558526 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTTAGCCTGTTTGATGCGTGCATTGCGC |
| Internal bar code: | CCGGTAGAGTATCGCGTCGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 713 |
| LEAP-Seq percent confirming: | 99.6488 |
| LEAP-Seq n confirming: | 1986 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCTTGTGAACTACGCTTG |
| Suggested primer 2: | GCTGGACGTACCCATCAAGT |