Insertion junction: LMJ.RY0402.162809_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.162809
Chromosome:chromosome 17
Location:6016787
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g740800 (1 of 5) PF06742//PF06863 - Protein of unknown function (DUF1214) (DUF1214) // Protein of unknown function (DUF1254) (DUF1254) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTGTGTGGCTCCCTTTCCCTAGACCAACG
Internal bar code:AAGGGGAGACTCGAGGGACATA

Confirmation - LEAP-Seq

LEAP-Seq distance:982
LEAP-Seq percent confirming:95.7831
LEAP-Seq n confirming:1749
LEAP-Seq n nonconfirming:77
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:ACACTCACGGAGAAGGTGCT
Suggested primer 2:TGCTCAGGGTCAGCTAGGTT