Insertion junction: LMJ.RY0402.162810_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.162810
Chromosome:chromosome 4
Location:3630774
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g228650 (1 of 18) 3.6.3.44 - Xenobiotic-transporting ATPase / Steroid-transporting ATPase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTGCCAAACCTCCTCCGCGCTCTTTCGGC
Internal bar code:TCGTACTTTCAATGGATATGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:67
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:150
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CCCTTTCACGGGCATAGTTA
Suggested primer 2:GGAGGTGTGGACAGAGGTGT