Insertion junction: LMJ.RY0402.162816_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.162816
Chromosome:chromosome 2
Location:285379
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g075050 PTA1 (1 of 10) IPR005828//IPR020846 - Major facilitator, sugar transporter-like // Major facilitator superfamily domain; Proton/phosphate symporter 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGGGCTGGCACGACTGGCACGACTTGGCC
Internal bar code:CAGGAGACGTCTCACAAGGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1143
LEAP-Seq percent confirming:90.1206
LEAP-Seq n confirming:1569
LEAP-Seq n nonconfirming:172
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:CGGTACCCTGATGTCGAGTT
Suggested primer 2:ACGCGCTTGATATTCAGCTT