| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.162819 |
| Chromosome: | chromosome 3 |
| Location: | 6740640 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g198000 | PPP15 | (1 of 2) PF00481//PF13672 - Protein phosphatase 2C (PP2C) // Protein phosphatase 2C (PP2C_2); Phosphoprotein phosphatase 2C-related | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTCGGTGGCCTGCAGGGACCTCGCCGGG |
| Internal bar code: | ATGGCACGTTAGATTTTATTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 551 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCAATGCGAAAAGTCGTG |
| Suggested primer 2: | ATGACGGTATGTCAGAGGGC |