Insertion junction: LMJ.RY0402.162873_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.162873
Chromosome:chromosome 9
Location:3223845
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g387652 GT90-41,GT90F41 GT90 family protein 41; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCTATGCAATGTCCATTGAGTCTTAACAG
Internal bar code:TCAATTCGAGTTTGGAGGGGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:914
LEAP-Seq percent confirming:99.9115
LEAP-Seq n confirming:1129
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:ACACACACACACACACACGC
Suggested primer 2:ATACTTGCTGAGGCCGCTTA