| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.162908 |
| Chromosome: | chromosome 8 |
| Location: | 373725 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358572 | (1 of 5) PF03094 - Mlo family (Mlo) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGGGAGCCCTCGTGCGAATGTGTGGGCC |
| Internal bar code: | CGCAAATGGCTTATTCCCCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1018 |
| LEAP-Seq percent confirming: | 96.3516 |
| LEAP-Seq n confirming: | 22316 |
| LEAP-Seq n nonconfirming: | 845 |
| LEAP-Seq n unique pos: | 126 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCCCAAACTACACCGAAT |
| Suggested primer 2: | TGAGACGAGGACACGAGATG |