| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.162949 |
| Chromosome: | chromosome 9 |
| Location: | 3709994 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g390949 | FAP140 | Flagellar Associated Protein 140 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGGCTCCAAGGCTGGCCTTCCAGGTGAC |
| Internal bar code: | CCCCGTCGGGATGCGCTACCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 272 |
| LEAP-Seq percent confirming: | 99.5807 |
| LEAP-Seq n confirming: | 475 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGAACGACTTACCTCGGC |
| Suggested primer 2: | AATCACCAATTTCTCGGCTG |