Insertion junction: LMJ.RY0402.162949_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.162949
Chromosome:chromosome 9
Location:3709994
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g390949 FAP140 Flagellar Associated Protein 140 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTGCACCACACCCATGCCAGAGTCAGTTC
Internal bar code:TCTAAGTGTTCTCTACCTCAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:639
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:917
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CAAGAACGACTTACCTCGGC
Suggested primer 2:AATCACCAATTTCTCGGCTG