| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.162970 |
| Chromosome: | chromosome 17 |
| Location: | 5874138 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g739850 | LRRC6,Seahorse,DNAAF11,MOT47 | (1 of 8) PF14580 - Leucine-rich repeat (LRR_9); Leucine-rich repeat-containing protein 6 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAACCTGCAGCGCTGCGAGAGCCTTAAAA |
| Internal bar code: | AGCGCATTGTTATTGAACTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 83 |
| LEAP-Seq percent confirming: | 99.2647 |
| LEAP-Seq n confirming: | 135 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGAGCCAAGGTGTATGGG |
| Suggested primer 2: | GGTTCCACAACGAAGCATTT |