| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.162971 |
| Chromosome: | chromosome 4 |
| Location: | 1167730 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g215750 | (1 of 1) K02180 - cell cycle arrest protein BUB3 (BUB3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCGCCGGGTGGGCAACAGGGCAGGGCGC |
| Internal bar code: | GCGAGTTACGGCGTCAATATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1126 |
| LEAP-Seq percent confirming: | 99.6954 |
| LEAP-Seq n confirming: | 7854 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 61 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGACATTGGTATTTGCCG |
| Suggested primer 2: | GTGTTTGTGCCCCTTCTCAT |