Insertion junction: LMJ.RY0402.163001_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.163001
Chromosome:chromosome 16
Location:1080102
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre16.g649550 (1 of 83) IPR029044 - Nucleotide-diphospho-sugar transferases intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCTTTCCGCCGCTCTTCTGACCCTCTCTG
Internal bar code:GCGTAGGTGCGTACAGGATCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:2
LEAP-Seq percent confirming:48.5619
LEAP-Seq n confirming:439
LEAP-Seq n nonconfirming:465
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AGTACGATGGACACGAAGGG
Suggested primer 2:CACACACCAGACACGAATCC