Insertion junction: LMJ.RY0402.163010_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.163010
Chromosome:chromosome 12
Location:5539185
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g531350 CGL62 putative cell cycle associated protein; (1 of 3) PF10497 - Zinc-finger domain of monoamine-oxidase A repressor R1 (zf-4CXXC_R1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACTCATCGAGTATTCACGCAGTCATGCCT
Internal bar code:GGTTGTCCGGACCTACCACAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:565
LEAP-Seq percent confirming:99.3485
LEAP-Seq n confirming:305
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CAAGGAGAAGGCTGTCGAAC
Suggested primer 2:CGCACTGTCAGAGCATCAAT