| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.163031 |
| Chromosome: | chromosome 16 |
| Location: | 1979703 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g656500 | ALA3 | (1 of 1) K01530 - phospholipid-translocating ATPase (E3.6.3.1); ATPase, phospholipid transporter | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAACCATCCAGCGACCGGCAGACAGGGTT |
| Internal bar code: | TGCGTCGGGGGTGACCGGCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 647 |
| LEAP-Seq percent confirming: | 99.8279 |
| LEAP-Seq n confirming: | 1160 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGGACCTGTAGCAAGCTG |
| Suggested primer 2: | GCAGGAGATTGAGTGGAAGC |