| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.163042 |
| Chromosome: | chromosome 9 |
| Location: | 3218285 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387615 | (1 of 3) PF10497 - Zinc-finger domain of monoamine-oxidase A repressor R1 (zf-4CXXC_R1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGATGCCAAGCGCCTGGGCTACAAGAGCGT |
| Internal bar code: | TGCACGGCTTGGGCGTGTTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 852 |
| LEAP-Seq percent confirming: | 99.7314 |
| LEAP-Seq n confirming: | 5940 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTTCACATAAGTGCCGTT |
| Suggested primer 2: | GGTTGGCAAGGTTTGACACT |