| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.163047 |
| Chromosome: | chromosome 9 |
| Location: | 1966439 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394850 | TEF24 | LrgB-like protein; (1 of 1) PTHR30249 - PUTATIVE SEROTONIN TRANSPORTER | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGGTTCAATCCACGTCCCGCCGCTTCCG |
| Internal bar code: | GCGTCGTGCAATCCCGTTAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 389 |
| LEAP-Seq percent confirming: | 99.8966 |
| LEAP-Seq n confirming: | 966 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTGTACCCATGCTGAGGG |
| Suggested primer 2: | CCGAAGTCTTGAAGCCAGTC |