| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.163050 |
| Chromosome: | chromosome_1 |
| Location: | 2005641 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g010832 | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCCATGCGAGAACGCGCCGAGCGGGTCGCC |
| Internal bar code: | TAGGTCTATCTCCCCTTACTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 866 |
| LEAP-Seq percent confirming: | 99.2282 |
| LEAP-Seq n confirming: | 7328 |
| LEAP-Seq n nonconfirming: | 57 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATTCAACAGCTCCACTGA |
| Suggested primer 2: | GGACCAACTTGCACTCCCTA |