| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.163059 |
| Chromosome: | chromosome_11 |
| Location: | 2539327 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre11.g476050 | ODA2,DHC15,DYHG | Flagellar outer arm dynein heavy chain gamma | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGGGTAGGCGCCGGCAATGTAGGCCGACAG |
| Internal bar code: | GCGGAGTCGTGTGCCCCGTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 932 |
| LEAP-Seq percent confirming: | 93.1993 |
| LEAP-Seq n confirming: | 3385 |
| LEAP-Seq n nonconfirming: | 247 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTCAGCTGGTTCTTGACC |
| Suggested primer 2: | TACTTTTCCACTTGCACCCC |