Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.163059 |
Chromosome: | chromosome_11 |
Location: | 2539359 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre11.g476050 | ODA2,DHC15,DYHG | Flagellar outer arm dynein heavy chain gamma | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTCCAACCTGTTTGAGGACATCAAGGGCCT |
Internal bar code: | TGGTTAGCGAAGCTTCTAATAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 780 |
LEAP-Seq percent confirming: | 81.8182 |
LEAP-Seq n confirming: | 27 |
LEAP-Seq n nonconfirming: | 6 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCTTCAGCTGGTTCTTGACC |
Suggested primer 2: | TACTTTTCCACTTGCACCCC |