| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.163063 |
| Chromosome: | chromosome 3 |
| Location: | 3822961 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g170600 | POC4 | Centriole proteome protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGCTGCAGCGTCTTTCTCGCGCCTGCCA |
| Internal bar code: | AGCGATTTTTTGTTCATGTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 207 |
| LEAP-Seq percent confirming: | 99.5671 |
| LEAP-Seq n confirming: | 230 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCCACTCACACCGTATGT |
| Suggested primer 2: | ATAGATATCATGCTGCGGGC |