| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.163091 |
| Chromosome: | chromosome_14 |
| Location: | 1868797 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g620600 | PHC2 | Cell wall protein pherophorin-C2 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGCATGCTCGGGCTATTTTCATGATTTCAG |
| Internal bar code: | GGAAAGATGGCGATAAGACAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 405 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 184 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCATCAGCCACTTGTGAA |
| Suggested primer 2: | GCCTTGGAGCAGCAGTAATC |