| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.163098 |
| Chromosome: | chromosome 10 |
| Location: | 5572412 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g459700 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAGCAGCAATGCCGGCGGCAGTAGCGGCA |
| Internal bar code: | CTGTGCCAAGATTCCCGGAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 350 |
| LEAP-Seq percent confirming: | 96.2264 |
| LEAP-Seq n confirming: | 51 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGTCGTTTGCATCGGGTT |
| Suggested primer 2: | GCACTAGCAGCAGTAGCACG |