| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.163139 |
| Chromosome: | chromosome_9 |
| Location: | 2811996 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g388850 | ACA1 | P-type ATPase/cation transporter, plasma membrane | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TAGCGATTGCGGGTGCGCCGGCCCTCGATG |
| Internal bar code: | GGTTCTCAAACCGACGCGACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 933 |
| LEAP-Seq percent confirming: | 97.2181 |
| LEAP-Seq n confirming: | 4578 |
| LEAP-Seq n nonconfirming: | 131 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCCTAAAGCTGGATGGTT |
| Suggested primer 2: | TCTAGGAAACGGCCAAAATG |