| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.163174 |
| Chromosome: | chromosome 17 |
| Location: | 5403649 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g736750 | (1 of 2) PTHR22640//PTHR22640:SF2 - UNCHARACTERIZED // STRUCTURAL MAINTENANCE OF CHROMOSOMES FLEXIBLE HINGE DOMAIN-CONTAINING PROTEIN 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTACCGAGCCCCTGGAGGTAGGCACCCG |
| Internal bar code: | AAGTGATGAAGTGCCACACAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1128 |
| LEAP-Seq percent confirming: | 98.7486 |
| LEAP-Seq n confirming: | 5208 |
| LEAP-Seq n nonconfirming: | 66 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCAGAGAGAACGAGCAGC |
| Suggested primer 2: | TGACGATGAGCTTCAACTGG |