Insertion junction: LMJ.RY0402.163176_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.163176
Chromosome:chromosome_11
Location:2408429
Confidence (%):58
Locus systematic id Locus common name Defline Orientation Feature
None

Insertion site details

Flanking sequence (orientation from cassette outwards):CTGTCATGTGCGTGACGAAGGAGGGGCCTG
Internal bar code:GACATATTTGCCGCCGTGTGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:553
LEAP-Seq percent confirming:99.6473
LEAP-Seq n confirming:1130
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:AATCGAGGAAGGAAAGCCAT
Suggested primer 2:GAAAGTGCAAGACAATCGCA